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doi: 10.2147/DDDT.S411211 272

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10.1186/s12943-020-01168-8 130 ZhangJ.ChenX.HongJ.TangA.LiuY.XieN.et al (2021)

If the medicine name or dose doesnt match what your healthcare provider prescribed, the medicine is expired, or the medicine is foamy, discolored, cloudy, or has crystals, dont use the syringe

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Library preparation For each sample, up to 200 ng of cDNA (as determined by PicoGreen quantification) was fragmented using a Covaris E220 Focused Ultrasonicator (Covaris) with the following parameters: 200 peak incident power, 25% duty cycle, 50 cycles per burst, and 10 s duration repeated 120 times